Why Neutrons get a wider angle on DNA and RNA to advance 3-D models

Why Neutrons get a wider angle on DNA and RNA to advance 3-D models

Oak Ridge National Laboratory (ORNL) to enable more precise computer simulation to acquire new information about the scientific DNA molecules and RNA molecules of the National Institute of Standards and Technology (NIST) and University of Maryland and interact with each other.

Are using neutron in Some from protein to viruses Solving three-dimensional structures of basic genetic material in the body will play an important role in the drug discovery and the development of important medical treatments.

Alexander Grechayev of NIST, who led the NIST team, said, “A better understanding of the structure and mobility of both RNA, RNA and RNA can help us to answer why and why drugs work and find out Help. ” Main talk at nuclear level “. The energy utility facility of the Ministry of Energy, located in OFNL, was researching the neutron dispersion in HFIR.

The team used HFIR’s BIO-SANS tool to operate a small, wide-angle neutron scattering, a technique that was previously not done on RNA in DNA samples and solutions due to limited experimental capacities.

“Until recently it was not possible to obtain a wide range of biomedical angles in the solution using the neutron scattering, and the OK ridge is the only place where you can do this kind of work,” Greeshche said.

The expansion of neutron dispersion capabilities in the solution is part of an evolved effort towards a more integrated approach to structural biology that connects crystalline studies, solution methods, and other experimental and computational techniques to increase the understanding of DNA and protein structures. .

Computer simulation of biomolecules was well informed by X-ray crystallography. The primary technique uses X-rays to determine the sequence of atoms in the “crystallized” sample for analysis.

To obtain high-quality data using this technique, generally diluted biological samples are concentrated in the solution and hardened in the crystal with a similar structure.

X-ray crystals work particularly well for solid biom with strong stable structures, but flexible biological molecules such as DNA and RNA, which adopt many “matches” or are less suitable for crystallization.

Within living cells, DNA and RNA can be transferred, change shape and can react differently to environmental effects such as pH or temperature, which is important to represent but it is difficult to describe.

Grazev said, “Pegas tightly closes the molecules tightly, limits their movements and want to see some structural information.”

In a solution, many techniques have been successfully implemented for DNA and RNA, including X-ray dispersion and NMR spectroscopy, which both produce significant data. However, there are significant differences between experimental scattering data and the best crystalline structures available for DNA and DNA.

The team switched to neutron to find out why.

“Rodrigo Acevedo of Maryland said,” Neutron interacts with biomolecules in different ways, so we can use them as an independent source of data.

While X-rays work well to identify heavy atoms such as carbon, oxygen, and phosphorus, neutrons are ideal for mild hydrogen atoms that are binding with DNA filaments for example. In addition, neutrons provide a benefit in biomolecules investigation because they are neither destructive nor harmful.

Using Bio-SNS tools in HFIR, researchers have been able to collect structural information in a solution that is not easily achieved by other experimental techniques.

The solution requires the use of high neutron flux and wide angle detectors to collect high-resolution dispersion patterns to detect atomic level structures of DNA and RNA.

Gresave says, neutron use is not uncommon for collecting structural information about biomolecules. In thin solutions, small organic molecular samples often produce noise dispersion patterns, making data analysis difficult.

Bio-SNS Tool Scientist, Volker Urban said, “HFIR’s Bio-SNS is one of the few neutron tools in the world which has the ability to catch small scattered and wide angles at the same time and combine global and local details. . ”

“We have been able to obtain some high-resolution data on the dispersion of neutrons, which have been collected not only on DNA and RNA, but also on broad biomolecules as well, from wide angles,” Greeshche said.

By combining new information gathered through the dispersion of neutron in the solution of X-ray dispersion and other data from MRI, the NIST-Maryland group expects to obtain a more comprehensive picture of DNA and RNA structures. RNA), in addition to the expansion of methods to identify particle structures with neutron-based technologies.


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